Fig. 6: ATF4 overexpression reverses the anti-tumor effect of METTL knockdown. | Cell Death Discovery

Fig. 6: ATF4 overexpression reverses the anti-tumor effect of METTL knockdown.

From: METTL1 coordinates cutaneous squamous cell carcinoma progression via the m7G modification of the ATF4 mRNA

Fig. 6

A, B Metabolic phenotypes of HSC-1 and A431 cells were determined by the both OCR and ECAR assays (n = 5). C Glucose uptake in HSC-1 and A431 cells were detected glucose assay (n = 5). D The ATP production was evaluated through ATP assay kit (n = 5). E Lactate levels in the medium were assessed (n = 5). F The protein levels of GLUT1, HK2 and LDHA were detected by western blot (n = 5). β-Tubulin was used for the normalization control. G MMP was measured by flow cytometry (n = 3). H The mitochondrial mass was measured by MitoTracker (n = 3). I EdU staining assay was used to assess cell proliferation (n = 3). J TUNEL staining of HSC-1 and A431 cells was performed (n = 3). K The mobility and invasiveness were assessed by trans-well migration assay and matrigel invasiveness measurement (n = 5), respectively. Data are shown as mean ± SEM. *P < 0.05, ***P < 0.001.

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