Fig. 7: ATF4 overexpression counteracted the inhibitory effects of METTL knockdown on tumor growth in vivo. | Cell Death Discovery

Fig. 7: ATF4 overexpression counteracted the inhibitory effects of METTL knockdown on tumor growth in vivo.

From: METTL1 coordinates cutaneous squamous cell carcinoma progression via the m7G modification of the ATF4 mRNA

Fig. 7

ATF4 overexpression promoted METTL1-deficient HSC-1 xenograft tumor growth (A) and reduced tumor weight (B). ATF4 overexpression promoted METTL1-deficient A431 xenograft tumor growth (C) and reduced tumor weight (D). E, F The protein levels of PCNA in tumor tissues were detected by IHC and western blot. The IOD of PCNA was calculated. β-Tubulin was used for the normalization control. The relative protein level was calculated. G The mRNA level of Pcna was evaluated by qRT-PCR. H The schematic diagram of oncogenic effects of METTL1-midiated m7G modification in cSCC through enhancing the mRNA stability of ATF4 and subsequently expression. Data are shown as mean ± SEM. n = 5. **P < 0.01, ***P < 0.001.

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