Table 2 The sequences for shRNA.
Name | Sequence |
|---|---|
shMETTL1-1 | GGTGTATACCATAACCGATGT |
shMETTL1-2 | CCCACATTTCAAGCGGACAAA |
shMETTL1-3 | GATGACCCAAAGGATAAGAAA |
The sequences of primers used for RT-qPCR (human) | |
 METTL1 F | 5’-GGCAACGTGCTCACTCCAA-3’ |
 METTL1 R | 5’-CACAGCCTATGTCTGCAAACT-3’ |
 BCL-2 F | 5’-GGTGGGGTCATGTGTGTGG-3’ |
 BCL-2 R | 5’-CGGTTCAGGTACTCAGTCATCC-3’ |
 BAX F | 5’-CCCGAGAGGTCTTTTTCCGAG-3’ |
 BAX R | 5’-CCAGCCCATGATGGTTCTGAT-3’ |
 ATF4 F | 5’-ATGACCGAAATGAGCTTCCTG-3’ |
 ATF4 R | 5’-GCTGGAGAACCCATGAGGT-3’ |
 HIF1α F | 5’-GAACGTCGAAAAGAAAAGTCTCG-3' |
 HIF1α R | 5’-CCTTATCAAGATGCGAACTCACA-3’ |
 MYC F | 5’-GGCTCCTGGCAAAAGGTCA-3’ |
 MYC R | 5’-CTGCGTAGTTGTGCTGATGT-3’ |
 STAT5A F | 5’- GCAGAGTCCGTGACAGAGG-3’ |
 STAT5A R | 5’-CCACAGGTAGGGACAGAGTCT-3’ |
 STAT3 F | 5’-CAGCAGCTTGACACACGGTA-3’ |
 STAT3 R | 5’-AAACACCAAAGTGGCATGTGA-3’ |
 SLC2A1 F | 5’-ATTGGCTCCGGTATCGTCAAC-3’ |
 SLC2A1 R | 5’-GCTCAGATAGGACATCCAGGGTA-3' |
 SLC2A3 F | 5’- GCTGGGCATCGTTGTTGGA-3’ |
 SLC2A3 R | 5’- GCACTTTGTAGGATAGCAGGAAG-3’ |
 PKM F | 5’-ATGTCGAAGCCCCATAGTGAA-3’ |
 PKM R | 5’-TGGGTGGTGAATCAATGTCCA-3’ |
 LDHA F | 5’-TTGACCTACGTGGCTTGGAAG-3’ |
 LDHA R | 5’-GGTAACGGAATCGGGCTGAAT-3’ |
 ENO1 F | 5’-TGGTGTCTATCGAAGATCCCTT-3’ |
 ENO1 R | 5’-CCTTGGCGATCCTCTTTGG-3’ |
 TUBULIN F | 5’-TGGACTCTGTTCGCTCAGGT-3’ |
 TUBULIN R | 5’-TGCCTCCTTCCGTACCACAT-3’ |
The sequences of primers used for RT-qPCR (mouse) | |
 Pcna F | 5’-TTGCACGTATATGCCGAGACC-3’ |
 Pcna R | 5’-GGTGAACAGGCTCATTCATCTCT-3’ |
 Tubulin F | 5’-CACCTGCAAGCCGGTCAAT-3’ |
 Tubulin R | 5’-TCCCCATGATAGGTCCCAGTG-3’ |
The sequences of primers used for plasmid construction. | |
 pCDNA3.1-ATF4 F | 5’-GGGAGACCCAAGCTGGCTAGCCTCGAGG CCACCATGACCGAGATGAGCTTCCTGAA-3’ |
 pCDNA3.1-ATF4 R | 5’-GTCATGGTCTTTGTAGTCC AACTCTCTTCTTCCCCCTTG-3' |
The sequences for siRNA | |
 si-ATF4#1 | 5’-CCAAAUAGGAGCCUCCCAUTT-3’ |
 si-ATF4#2 | 5’-AUGGGAGGCUCCUAUUUGGTT-3' |
