Fig. 6: Effects of statins and scDb-hERG1-β1 treatments on PDAC cell vitality, proliferation and migration. | Cell Death Discovery

Fig. 6: Effects of statins and scDb-hERG1-β1 treatments on PDAC cell vitality, proliferation and migration.

From: Targeting the hERG1/β1 integrin complex in lipid rafts potentiates statins anti-cancer activity in pancreatic cancer

Fig. 6

A Proliferation curve of PANC-1 CTR and treated with IC50 of SIM for 24 h, 48 h, 72 h and 96 h. Data are presented as mean values ± s.e.m. (n = 3). B Representative Calcein/PI images of PANC-1 CTR and treated with IC50 of SIM, scDb-hERG1-β1 and IC50 of SIM + scDb-hERG1-β1 for 24 h, 48 h, 72 h and 96 h (left panel) (scale bar: 100 μm) and cell live index (%) graph (right panel). Data are presented as mean values ± s.e.m. (n = 3). C Facs plots of cell cycle of PANC-1 cells treated with with IC50 of SIM, scDb-hERG1-β1 and IC50 of SIM + scDb-hERG1-β1 for 24 h. SIM vs CRT: p = 0.004 (G1); p = 0.021 (S); p = 0.038 (G2/M). SIM vs scDb-hERG1-β1: p = 0.005 (G1); p = 0.038 (S); p = 0.039 (G2/M). SIM+ scDb-hERG1-β1 vs CRT: p = 0.0002 (G1); p = 0.001 (S); p = 0.0001 (G2/M). Data are presented as mean values ± s.e.m. (n = 3). D Graph of percentage live cells, PANC-1 (left panel) and MiaPaCa2 (right panel) cells, treated for 24 h with IC50 of SIM, FLUVA, LOVA, ATOR, scDb-hERG1-β1 and combination of each statin with scDb-hERG1-β1 are reported. Data are presented as mean values ± s.e.m. (n = 3). E Lateral motility experiments onto FN were performed on PANC-1 cells treated with SIM, FLUVA, LOVA, ATOR, scDb-hERG1-β1 (at IC50 values for 90 min) and combinations of each statin and scDb-hERG1-β1 (at IC50 values for 90 min). Representative images are reported in the left panel. The motility is reported as graph of percentage of cell motility in the right panel. Scale bar: 100 µm. Data are presented as mean values ± s.e.m. (n = 3). F Lateral motility experiments onto FN were performed on MiaPaCa2 cells treated with SIM, FLUVA, LOVA, ATOR, scDb-hERG1-β1 (at IC50 values for 90 min) and combinations of each statin and scDb-hERG1-β1 (at IC50 values for 90 min). Representative images are reported in the left panel. The motility is reported as graph of percentage of cell motility in the right panel. Scale bar: 100 µm. Data are presented as mean values ± s.e.m. (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 (one-way ANOVA). CTR control, SIM Simvastatin, FLUVA Fluvastatin, LOVA Lovastatin, ATOR Atorvastatin.

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