Fig. 2: Loss of p63γ shortens the lifespan of tumor-free mice and promotes cellular senescence.
From: TAp63γ is the primary isoform of TP63 for tumor suppression but not development
A Kaplan–Meier survival curves of tumor-free WT (n = 40), TAp63+/− (n = 9), p63γ+/− (n = 9) and p63γ−/− (n = 17) mice. B SA-β-gal assay was performed with kidney tissues from WT, p63γ+/− and p63g−/− mice. C RT-PCR was performed to measure the levels of p63g and GAPDH transcripts in WT, p63γ+/− and p63γ−/− MEFs isolated from 13.5-day-old embryos from the same litter. D SA-b-gal assay was performed to measure the percentage of senescence cells from WT, p63γ+/− and p63γ−/− MEFs. * indicated p < 0.05 by Student’s t test. E Western blot analysis was performed to measure the levels of p130, PML and actin in WT, p63γ+/− and p63γ−/− MEFs used in (C). The relative fold change of protein was shown below each lane.
