Fig. 2: Inhibition of osteoclast differentiation by Drg2 downregulation in BMMs is associated with inhibition of Rac1 activation. | Cell Death Discovery

Fig. 2: Inhibition of osteoclast differentiation by Drg2 downregulation in BMMs is associated with inhibition of Rac1 activation.

From: Rac1-dependent regulation of osteoclast and osteoblast differentiation by developmentally regulated GTP-binding 2

Fig. 2

A Control siRNA or Drg2 siRNA transfected-BMMs were cultured in M-CSF and RANKL for 3 days. The mRNA levels were analyzed using quantitative PCR. N = 3. B Control siRNA or Drg2 siRNA transfected-BMMs were serum-starved and stimulated using RANKL for the indicated times. Protein levels were assessed using Western immunoblotting. N = 3. C Control siRNA or Drg2 siRNA transfected-BMMs were stimulated with M-CSF and RANKL. Whole-cell lysates were incubated with GST fusion proteins containing the PAK1 RBD for the pulldown assay. Protein levels were assessed using Western immunoblotting. N = 3. D Control siRNA or Drg2 siRNA transfected-BMMs were cultured in M-CSF and RANKL in the absence or presence of NSC 23766. The numbers of osteoclasts were determined after TRAP staining. N = 3. Scale bar, 200 µm. Data represent the mean ± SD of triplicate samples. #P < 0.05, *P < 0.01 vs. control.

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