Fig. 3: Effect of PS1 knockdown on APP and TDP43 expression.

A Effect of whether to knock down PS1 in HeLa cells on APP and TDP43 expression. It examines the impact of TDP43 overexpression on APP expression in HeLa cells following transfection with NC and siRNA. Additionally, it investigates the alterations in TDP43 (panel 1 and 2) and APP (panel 4) expression in HeLa cells after backfilling with PS1. Relative protein level was calculated respectively by band intensity against GAPDH unless noted otherwise in this study. C Effect of whether to knock down PS1 in NSC34 cells on APP and TDP43 expression. The western blot of TDP43 (panel 1) and APP (panel 3) expression in NSC34 cells following siRNA knockdown of PS1 for 24 h, followed by transfection of TDP43 and PS1. E The effect of transfection of TDP43 and PS1 on TDP43 and APP expression in WT MEF cells following knockdown of PS1. Mice MEF cells were extracted from WT female mouse embryos at 11.5 days of gestation and subsequently transfected with siRNA for 24 h to knock down the expression of PS1 in WT MEF cells. Following this, the cells were transfected with TDP43 and PS1, the expression of TDP43 (panel 1) and APP (panel 3) was detected after 24 h, and the results were compared with those of the NC group. G siRNA-mediated knockdown of PS1 in primary neuron cells. NGF-induced differentiation of WT MEF cells into primary neuron cells. Then siRNA knockdown of PS1 in primary neuron cells was followed by transfection with TDP43 and PS1. The expression of TDP43 and APP was detected by WB. B and D Grayscale values of total TDP43, APP and PS1. F and H Grayscale values of total TDP43 and APP. The data are the mean ± SD of at least three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant).