Fig. 5: Effects of TDP43 and its three mutants on PS1/γ-secretase substrates.
From: TDP43 is a newly identified substrate for PS1, enhancing the expression of APP following cleavage
A–C. TDP43 and its mutants increased APP levels in HeLa and NSC34 cells, while the A315T mutant affected E-cadherin cleavage. HeLa cells (A) and NSC34 (C) were seeded in 35-mm dishes and transfected with 4 μg of either LacZ, TDP43-WT, -N390D, -G348C, or -A315T for 24 h before samples were collected, and levels of APP (Panel 3) and E-cadherin (Panel 4) were detected by western blotting. B, D The grayscale values for APP and E-cadherin in HeLa and NSC34. The grayscale values for APP and E-cadherin in HeLa (B) and NSC34 (D) are the mean ± SD of at least three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant).
