Fig. 6: Interaction between TDP43 and PS1 increases Aβ levels.
From: TDP43 is a newly identified substrate for PS1, enhancing the expression of APP following cleavage
A Interaction of PS1 and its mutants with TDP43 increases Aβ levels. HeLa cells were transfected with LacZ, TDP43, and PS1, as well as co-transfected with TDP43 and PS1-WT or TDP43 and PS1-mutants (D257A and D385A). Aβ levels were detected using immunofluorescence, with DAPI marking cell nuclei and FITC marking Aβ (green fluorescence). The merge images show the integration of both channels. C Interaction of TDP43-WT, -N390D, and -G348C with PS1 promotes Aβ production, while TDP43-A315T mutant inhibits Aβ production. HeLa cells were transfected with LacZ, TDP43, PS1, or co-transfected with PS1 and TDP43-WT, -N390D, -G348C, or -A315T. Aβ levels were detected via immunofluorescence, with DAPI marking cell nuclei and FITC marking Aβ (green fluorescence). The merge images show the integration of both channels. B, D Statistical graphs illustrating Aβ fluorescence intensity. The fluorescence intensity of Aβ and the total area of all cells in the DAPI channel were calculated using the ImageJ software. The ratio of Aβ fluorescence intensity to the total area of the cells is the average fluorescence intensity of Aβ. Date are the mean ± SD of at least three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant).
