Fig. 4: NINJ1 is activated in pyroptotic bEC. | Cell Death Discovery

Fig. 4: NINJ1 is activated in pyroptotic bEC.

From: NINJ1-mediated plasma membrane rupture of pyroptotic endothelial cells exacerbates blood-brain barrier destruction caused by neutrophil extracellular traps in traumatic brain injury

Fig. 4

A Representative image of immunofluorescence staining of NINJ1 (green) in hCMECs. Nuclei were stained with DAPI (blue). The white asterisk indicates the cluster of NINJ1. Scale bars = 50 μm. B, C Western blotting and quantification of NINJ1 in hCMECs treated with siNC or siNINJ1 (n = 6). D Quantification of the release ratio of the PMR marker LDH in the supernatant medium of hCMECs, in which the siNC and siNINJ1 groups were both treated with LPS+nigericin (n = 5). E Quantification of the concentration of HMGB1 in the supernatant of hCMECs, in which the siNC and siNINJ1 groups were both treated with LPS+nigericin (n = 6). F, G Western blotting and quantification of NINJ1 in the cortex 3 days after TBI (p = 0.1486, n = 6). H Representative immunofluorescence staining of CD31 (green) and NINJ1 (red) in the cortex. Nuclei were stained with DAPI (blue). The white asterisk indicates colocalization of CD31 and NINJ1. Scale bar = 50 μm, scale bar on the enlarged images = 10 μm. Data were presented as the mean ± SD, ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001.

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