Fig. 5: Knockdown of NINJ1 mitigates neutrophil infiltration and NET formation. | Cell Death Discovery

Fig. 5: Knockdown of NINJ1 mitigates neutrophil infiltration and NET formation.

From: NINJ1-mediated plasma membrane rupture of pyroptotic endothelial cells exacerbates blood-brain barrier destruction caused by neutrophil extracellular traps in traumatic brain injury

Fig. 5

A–D Western blotting and quantification of NINJ1 and TJPs (ZO-1 and occludin) in the cortex of mice treated with AAV-TIE-shNC or AAV-TIE-shNINJ1 (n = 6). E Quantification of the concentration of HMGB1 in the plasma 3 days after TBI (n = 6). F–I Western blotting and quantification of the neutrophil (MPO and NE) and NET-specific (citH3) markers in the cortex 3 days after TBI (n = 6). J, K Representative image of immunofluorescence staining of citH3 (green) and NE (red) and quantification of NE+ citH3+ cells in the cortex 3 days after TBI. Nuclei were stained with DAPI (blue). Scale bar = 50 μm, scale bar on the enlarged images = 10 μm. Data were presented as the mean ± SD, ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001.

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