Fig. 6: Knockdown of NINJ1 ameliorates BBB destruction by alleviating bEC pyroptosis after TBI. | Cell Death Discovery

Fig. 6: Knockdown of NINJ1 ameliorates BBB destruction by alleviating bEC pyroptosis after TBI.

From: NINJ1-mediated plasma membrane rupture of pyroptotic endothelial cells exacerbates blood-brain barrier destruction caused by neutrophil extracellular traps in traumatic brain injury

Fig. 6

A–E Western-blotting and quantification of pyroptosis-related proteins (GSDMD-N, caspase-1 p20, caspase-1 p10, and NLRP3) in the cerebrovascular component of the cortex 3 days after TBI (D, p = 0.2771) (n = 6). F, G Representative image of immunofluorescence staining of CD31 (green) and NLRP3 (red) and quantification of the expression of NLRP3 in the cortex 3 days after TBI (n = 3). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. H–J Western blotting and quantification of tight junction proteins (ZO-1 and occludin) in the cortex 3 days after TBI (n = 6). K, L Representative image of double immunofluorescence staining of CD31 (green) and ZO-1 (red) and quantification of the percentage of ZO-1-covered area (%CD31 area) in the cortex 3 days after TBI (n = 3). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. M Representative images of brain tissue from the indicated treatment groups 3 or 7 days after TBI. The blue area indicates extravasation of Evans blue dye. N, O Quantification of leaked Evans blue dye in the ipsilateral cerebral hemisphere of mice from the indicated groups (n = 6). Data were presented as the mean ± SD, ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001.

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