Fig. 7: Knockdown of NINJ1 improves neurological outcomes after TBI. | Cell Death Discovery

Fig. 7: Knockdown of NINJ1 improves neurological outcomes after TBI.

From: NINJ1-mediated plasma membrane rupture of pyroptotic endothelial cells exacerbates blood-brain barrier destruction caused by neutrophil extracellular traps in traumatic brain injury

Fig. 7

A, B Western blotting and quantification of apoptosis-related protein caspase-3 p19 in the cortex 3 days after TBI (n = 6). C, D Representative image of immunofluorescence staining of NeuN (green) and cleaved caspase-3 (red) and quantification of cleaved caspase-3-postive neurons cells in the cortex 3 days after TBI (n = 3). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. E Quantification of neurons in the cortex 3 days after TBI (P = 0.2128, n = 3). F mNSS neurological function scores were assessed 1 day to 1 week after TBI in different groups of mice (n = 6). Data were presented as the mean ± SD, ns not significant, when the TBI + AAV-shCTRL group was compared to the sham group, ###p < 0.001, when the TBI + AAV-shNINJ1 group was compared to the TBI + AAV-shCTRL group, *p < 0.05, **p < 0.01, ***p < 0.001.

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