Fig. 2: DDR1-IN-1 resulted in the accumulation of LC3B-II in MPNST cells. | Cell Death Discovery

Fig. 2: DDR1-IN-1 resulted in the accumulation of LC3B-II in MPNST cells.

From: Discoidin domain receptor inhibitor DDR1-IN-1 induces autophagy and necroptotic cell death in malignant peripheral nerve sheath tumor

Fig. 2

The total protein lysates of (A) S462 and (B) STS26T MPNST cells treated with 0-10 μM DDR1-IN-1 for 24 hours, as well as (C) STS26T cells treated with 10 μM DDR1-IN-1 for 0-48 hours were extracted. Western blotting was performed to reveal the expression levels of various cell death-related proteins that participated in the autophagy or apoptosis pathway. The red arrowheads point out the cleaved PARP. D Autophagy inhibitor, 3-MA (5 mM), was co-treated separately with 10 μM DDR1-IN-1 to S462 cells for 24 hours. Total protein was extracted and the cell death-related markers were demonstrated through western blotting. The cleaved-Caspase3 is pointed out by the red arrow. E The ratio of LC3B-II to GAPDH was calculated and compared between each group (n = 3), and (F) the ratio of LC3B-I to LC3B-II was calculated and compared between each group (n = 3). The ratio is plotted in bar chart and the p values are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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