Fig. 3: RS-35d, S-35d and R-35d differentially influence cancer hallmarks of tumour aggressiveness. | Cell Death Discovery

Fig. 3: RS-35d, S-35d and R-35d differentially influence cancer hallmarks of tumour aggressiveness.

From: Investigating synthetic lethality and PARP inhibitor resistance in pancreatic cancer through enantiomer differential activity

Fig. 3

Evaluation of BxPC-3 cell proliferation (a), migration (b) and apoptosis (c) after exposure to 40 µM RS-35d, 20 µM S-35d or 20 µM R-35d alone or in combination with 10 µM OLA. a Representative images of BxPC-3 colonies and analysis of the corresponding colony rate (expressed as % of CTRL). b Representative images of BxPC-3 wound-healing scratches and analysis of the corresponding migration rate (expressed as % of the wound area at t = 0 h). c Representative dot-plots of PI- and FITC-Annexin V-stained BxPC-3 cells analysed through flow cytometry and analysis of the measured apoptotic cells (%). Results are expressed as mean ± SD (n = 3). Statistical analysis was performed with one-way ANOVA followed by Tuckey’s multiple comparison test, §p < 0.05 or §§§p < 0.001 vs CTRL; #p < 0.05, ##p < 0.01 or ###p < 0.001 vs 10 µM OLA; *p < 0.05, **p < 0.01 or ***p < 0.001 vs RAD51-BRCA2 inhibitor alone.

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