Fig. 6: RS-35d proteomic profile in BxPC-3 cells.
a Volcano plot of control vs RS-35d up- (red) and downregulated (green) proteins in BxPC-3 cells, p-value < 0.05 and log2(fold change) > 1 or < −1 were used as significance cut-off. b Venn diagram of RS-35d up- and downregulated common proteins with RAD51 interactome and ATM/ATR/DNA-PK common interactomes; Western blot analysis of FANCD2, FANCI and RPA3 expression in BxPC-3 cells treated with 40 µM RS-35d, 20 µM S-35d or 20 µM R-35d for 24 h. The images are representative Western blots. Results are normalised over β-actin expression and expressed as mean ± SD (n = 3). Statistical analysis was performed with two-way ANOVA followed by Dunnett’s multiple comparison test, with **p < 0.01 vs CTRL. c Gene Ontology (GO) cellular component, molecular functions and biological processes enriched from the analyses of the up- (red) and downregulated (green) proteins by RS-35d. d STRING functional analysis of up-regulated proteins by RS-35d. Proteins were clustered using Markov clustering (MCL), with default inflation parameter = 3; the thickness of lines representing protein-protein interactions indicates the strength of data support.
