Fig. 1: POLA2 downregulation in the wing imaginal disc causes replication stress.

A Confocal image of a wing imaginal disc expressing GFP under the control of ap-Gal4. In the merge, DAPI and GFP are shown in blue and green, respectively. Scale bar, 100 µm. B, C Confocal images of the wing pouch of ap > GFP (B) and ap > GFP + POLA2-i (C) discs labeled with EdU. In the merge, DAPI, GFP, and EdU are shown in blue, green, and grayscale, respectively. Scale bars, 10 µm. D, E Quantification of mean EdU intensity (D; n = 16, 24) and number of EdU-positive cells per area (E; n = 16, 24) in the dorsal compartment of the genotypes indicated in B and C. Data shown are mean ± SD. Statistical analyses were performed using a two-tailed unpaired t-test (D) and a two-tailed unpaired t-test with Welch’s correction (E). F Confocal image of the wing pouch of a wild-type disc carrying an RPA1-GFP sensor. A magnification is shown in F'. In the merge, DAPI and RPA1-GFP are shown in blue and green, respectively. Scale bars, 10 µm. G Confocal image of the wing pouch of an ap > POLA2-i disc carrying an RPA1-GFP sensor and labeled with EdU. A magnification of the dorsal compartment is shown in G'. In the merge, DAPI, RPA1-GFP, and EdU are shown in blue, green, and grayscale, respectively. Scale bars, 10 µm.