Fig. 7: Oncogenic traits in tissues with POLA2 downregulation and apoptosis suppression.

A-C Confocal images of ap > p35 + GFP discs (A) and ap > p35 + GFP + POLA2-i discs induced for either 4 days (B) or 7 days (C). DAPI and GFP are shown in blue and green, respectively. Scale bars, 100 µm. D-F Confocal images of ap > p35 + GFP discs (D) and ap > p35 + GFP + POLA2-i discs induced for 7 days (E, F) stained with anti-Dlg. Yellow arrows indicate clusters of GFP-positive cells that appear to detach from the main GFP-positive tissue. In the merge, DAPI, GFP, and Dlg are shown in blue, green, and red, respectively. Scale bars, 10 µm. G, H Confocal images of the wing pouch of ap > p35 + GFP (G) and ap > p35 + GFP + POLA2-i (H) discs induced for 4 days and stained with anti-MMP1 and anti-pJNK. Yellow arrows indicate dorsal GFP-positive cells that are located in the ventral compartment. In the merge, DAPI, GFP, MMP1, and pJNK are shown in blue, green, red, and grayscale, respectively. Scale bars, 10 µm. I, J Confocal images of mitotic cells (yellow arrows) stained with anti-PH3 in ap > p35 + GFP discs (I) and ap > p35 + GFP + POLA2-i discs induced for 5 days (J). In the merge, DAPI, GFP, and PH3 and shown in blue, green, and grayscale, respectively. Scale bars, 10 µm. K-N Confocal images of ap > p35 + GFP discs (K), ap > p35 + GFP + POLA2-i discs induced for 5 days (L), and ap > p35 + GFP + POLA2-i+stg discs induced for 5 and 10 days (M and N, respectively) stained with anti-PH3. Note that images in K–M were re-sized to match the scale bar in all panels. Magnifications of all discs are shown in K'–N'. In the merge, DAPI, GFP, and PH3 are shown in blue, green, and grayscale, respectively. Scale bars, 100 µm. In this figure, ap > p35 + GFP + POLA2-i discs with induction lengths of 4 or more days spent 7 days at 18 °C prior to induction.