Fig. 1: dCK knockdown promoted radiosensitivity of HeLa cells. | Cell Death Discovery

Fig. 1: dCK knockdown promoted radiosensitivity of HeLa cells.

From: HSP90 regulates dCK stability and inhibits ionizing radiation-induced ferroptosis in cervical cancer cells

Fig. 1

A Comparison of dCK expression levels between CESC tumors and normal tissues. B Kaplan–Meier curves of RFS in cervical cancer according to the dCK expression levels. C The expression level of dCK in HeLa cells after 8 Gy of IR in different time. D The knockdown efficiency of dCK was determined by Western blot in HeLa cells at 48 h after exposure to 8 Gy of IR. E The cell viability of sh-Control and sh-dCK HeLa cells after IR were detected by CCK-8 assay. F The radiosensitivity of sh-Control and sh-dCK HeLa cells were detected by colony formation assay. G Survival fraction with multi-target single-hit model. (H-I) Colony formation assay in sh-Control and sh-dCK HeLa cells that were pretreated with 20 μM ferrostatin-1 (fer-1), 20 μM Z-VAD-fmk (Z-VAD) or 10 μM chloroquine (CQ) or DMSO for 2 h after 4 Gy of IR. Error bars are means ± SD, n = 3 independent repeats. ns, non significance; *p < 0.05; **p < 0.01; ***p < 0.001. P values were calculated by using two-tailed unpaired student’s t-test.

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