Fig. 3: dCK regulated the mRNA level and protein stability of SLC7A11.

A Western blotting in HeLa cells with sh-Control and sh-dCK. B Evaluation of SLC7A11-expressing sh-Control and sh-dCK HeLa cells’ lipid peroxidation. C Evaluation of cell death in HeLa cells expressing sh-Control and sh-dCK and expressing SLC7A11. D Western blot analysis to identify the dCK and SLC7A11 interaction. E After cycloheximide (CHX) treatment, the half-life of the endogenous SLC7A11 protein, which had been lowered by dCK knockdown, was examined. F HeLa cells with dCK knockdown and overexpression subjected to qRT-PCR analysis. G, H An illustration of the human SLC7A11 gene schema. The purple and green triangles, respectively, represent the two AAREs (AARE-F and AARE-R) and the putative AREs (ARE-pro and ARE-int). I Scatter plots for TCGA data correlation analysis between ESR1 and dCK. J qRT-PCR analysis in HeLa cells with sh-Control and sh-dCK. K The effect of ESR1 overexpression on the promoter activity of SLC7A11 was investigated using the promoter dual-luciferase reporter assay.The error bars represent means ± SD based on three independent repeats. * Denotes non-significance; **p < 0.01; ***p < 0.001. Using a two-tailed unpaired student’s t-test, P values were determined.