Fig. 6: dCK could rescue HSP90 inhibitor-induced ferroptosis with or without IR in HeLa cells. | Cell Death Discovery

Fig. 6: dCK could rescue HSP90 inhibitor-induced ferroptosis with or without IR in HeLa cells.

From: HSP90 regulates dCK stability and inhibits ionizing radiation-induced ferroptosis in cervical cancer cells

Fig. 6

A The degree of HSP90 expression in HeLa cells following 8 Gy of IR at various times. B, C The assessment of cell death in sh-Control and sh-HSP90 HeLa cells 48 h following an 8 Gy IR exposure. D, E The assay for colony formation in sh-Control and sh-HSP90 HeLa cells that underwent 2 h of pretreatment with 4 Gy IR or 20 μM ferrostatin-1 (fer-1). F, G Evaluation of cell death in sh-Control and sh-HSP90 HeLa cells exposed to 8 Gy of IR for 48 h, either with or without dCK expression. H–L Tumor volumes, tumor weights and body weights of mice were taken and measured after 17AAG (50 mg/kg) or IR (8 Gy×3) treatment. M Western blot analysis of animal tumour tissues. The error bars show the means ± SD from three independent repeats. p < 0.05 for *, p < 0.01 for **, and p < 0.001 for ***. Using a two-tailed unpaired student’s t-test, P values were determined.

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