Table 4 The specifics of the primer sets used in the study.

From: Ursodeoxycholic acid prompts glycolytic dominance, reductive stress and epithelial-to-mesenchymal transition in ovarian cancer cells through NRF2 activation

Gene name

Primers (5′-3′)

Length (bp)

Specificity check

Alignments (Sequence ID)

Query coverage 100%

Percent identity 100%

Primer location

Exon intron boundary

Calibration curve

Efficiency

NRF2

FW: TGAGCAAGTTTGGGAGGAGCT

REV: ACTGGTTGGGGTCTTCTGTGG

245

BLAST

NM_001313903.2

FW 484-504

REV 728-708

YES

Slope: −3.428

YIntercept: 28.11

Error = 0.0735

1.958

NM_001313902.2

FW 613-633

REV 857-837

NM_006164.5

FW 703-723

REV 947-927

NM_001313904.1

FW 1165-1185

REV 1409-1389

NM_001313901.1

FW 1202-1222

REV 1446-1426

NM_001313900.1

FW 1110-1130

REV 1354-1334

NM_001145413.3

FW 1215-1235

REV 1459-1439

NM_001145412.3

FW 1236-1256

REV 1480-1460

TCF7L2

FW: ACGTACAGCAATGAACACTTCAC

REV: GGCGATAGTGGGTAATACGG

128

BLAST

NM_001198530.2

FW 952-974

REV 1079-1060

YES

Slope: -3.699

YIntercept: 29.42

Error = 0.204

1.863

NM_001146283.2

FW 1195-1217

REV 1322-1303

NM_001349870.2

FW 778-800

REV 905-886

NM_001146285.2

NM_001198528.2

NM_001146286.2

NM_001198527.2

NM_001198526.2

NM_001146284.2

NM_001198525.2

NM_001198529.2

NM_030756.5

FW 1054-1076

REV 1181-1162

NM_001367943.1

NM_001146274.2

NM_001363501.2

NM_001198531.2

FW 1123-1145

REV 1250-1231

NM_001349871.1

FW 310-332

REV 437-418

PPIA

FW: GTCTCCTTTGAGCTGTTTGCAGAC

REV: CTTGCCACCAGTGCCATTATG

171

BLAST

NM_021130.5

FW 102-125

REV 272-252

YES

Slope: -3,485

YIntercept: 25.54

Error= 0.0366

1.936

RPLP0

FW: CCATTGAAATCCTGAGTGATGTG

REV: GTCGAACACCTGCTGGATGAC

131

BLAST

NM_053275.4

FW 589-611

REV 719-699

YES

Slope: -3.552

YIntercept: 30.65

Error= 0.0548

1.912

NM_001002.4

FW 529-551

REV 659-639

  1. Reporting is made in accordance with the QIME guideline [97, 98].
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