Fig. 5: DZN induces apoptosis in testicular cells (GC-1 spg, TM3, and TM4). | Cell Death Discovery

Fig. 5: DZN induces apoptosis in testicular cells (GC-1 spg, TM3, and TM4).

From: Diazinon induces testicular dysfunction and testicular cell damage through increased reactive oxygen species production in mouse

Fig. 5

A Detection of in situ DNA fragmentation using a TUNEL assay. TUNEL-positive nuclei (red fluorescent) increased in a dose-dependent manner in DZN-treated GC-1 spg, TM3, and TM4 cells. Scale bar = 100 µm. The graphs present Tunnel/DAPI rate analysis as a percentage (mean ± SD, n = 5). B Representative flow cytometry plots for Annexin V (FITC) and propidium iodide (PI) stained cells. The graphs present apoptosis rate analysis as a percentage of apoptotic cells number (mean ± SD, n = 5). Asterisks indicate significant differences between the treatment and control groups at **p < 0.01 and ***p < 0.001.

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