Fig. 6: Effects of DZN on mitochondrial ROS production on testicular cells (GC-1 spg, TM3, and TM4). | Cell Death Discovery

Fig. 6: Effects of DZN on mitochondrial ROS production on testicular cells (GC-1 spg, TM3, and TM4).

From: Diazinon induces testicular dysfunction and testicular cell damage through increased reactive oxygen species production in mouse

Fig. 6

A Each cell was double stained with CellROX® FITC and MitoTracker Red to measure oxidative stress levels. CellROX® staining indicates oxidative stress, whereas the MitoTracker Red CMXRos signal indicates changes in mitochondrial membrane potential. Scale bar = 50 μm. B Flow cytometry dot plots for GC-1 spg, TM3, and TM4 cells stained with CellROX® for quantification. Histograms of ROS production obtained by flow cytometry after CellROX® staining of DZN-treated GC-1 spg, TM3, and TM4 cells. C Graph presents the quantitative change of CellROX in each group, and data are presented as the mean ± SD of five independent replicates. Asterisks indicate significant differences between the treatment and control groups at *p < 0.05 and ***p < 0.001.

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