Fig. 2: M2 receptor selective activation inhibits cell growth in hdASCs. | Cell Death Discovery

Fig. 2: M2 receptor selective activation inhibits cell growth in hdASCs.

From: Acetylcholine muscarinic M2 receptor maintains human Schwann-like adipose-derived phenotype in the absence of differentiating medium

Fig. 2

A M2 activation decreased cell growth after 72 h of treatment up to 7 days [P < 0.01(**), and P < 0.0001(****); n = 4]. Although a significant difference between muscarine treatment and untreated cells after 5 days of exposure [P < 0.0001(****); n = 4], muscarine treatment did not affect cell growth and cell number was comparable to control after 7 days. B After APE withdrawal from the culture media, a significant recovery of cell growth was observed. Recovery analysis suggests that the inhibition of M2-mediated cell growth is reversible [P < 0.0001(****); n = 4]. C, D Live-dead assay: APE and muscarine treatments had any effects on cell survival [live cells = green; dead cells = red; scale bar = 400 µm; P < 0.001(***); n = 4] while the reduction of live cells confirms a decrease of cell proliferation-APE induced.

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