Fig. 4: M2 selective activation inhibits cell migration.

A Pictures were taken at time 0 and after 6 h from the scratch. B The graph reported the distance traveled (µm) in 6 h; APE treatment significantly decreased cell migration (distance traveled: 40.7 ± 0.82 µm, ****P < 0.0001, n = 4); while the non-selective muscarinic receptors agonist, muscarine, increased cell migration (distance traveled: 76.29 ± 1.35 µm, ****P < 0.0001, n = 4). Scale bar = 400 µm.