Fig. 5: Thr440 O-GlcNAcylation of NONO promotes cancer cell invasion, colony formation, and proliferation. | Cell Death Discovery

Fig. 5: Thr440 O-GlcNAcylation of NONO promotes cancer cell invasion, colony formation, and proliferation.

From: O-GlcNAcylation of NONO regulates paraspeckle component assembly and contributes to colon cancer cell proliferation

Fig. 5

A WST-8 assay was performed to compare cell proliferation between NONO knockout, NONO WT-expressing, or NONO T440A-expressing HCT116 cells (n = 3 per condition). B Transwell invasion assay was performed and invaded cells were measured by WST-8 assay (n = 3 per condition). C NONO knockout, NONO WT-expressing, or NONO T440A-expressing HCT116 cells were cultured for soft agar colony formation assay. Colony formation was examined under a light microscope. Colony formation was measured by using a 485/520 nm filter set (n = 5 per condition). D BALB/c nude mice (n = 6 per group) were injected with 1 × 107 of the indicated cells subcutaneously. Tumor volume and weight were measured 60 days after injection. Data are presented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA with Tukey’s multiple comparisons test.

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