Fig. 1: Nuclear FBP1 is degraded through the ubiquitin-proteasome pathway.

A Western blot was performed to analyze FBP1 expression with the treatment of DMSO, MG132 (10 μM), or NH4Cl (10 mM) in 786-O cells. B Immunoprecipitation and Western blot with the indicated antibodies were performed to determine the level of FBP1 ubiquitylation in HEK293T cells. C Western blot was performed to assess the cytoplasmic and nuclear expression of FBP1 after the treatment of DMSO, MG132 (10 μM) or NH4Cl (10 mM) in 786-O cells.