Fig. 3: CUL4B degrades the FBP1 protein in the nucleus. | Cell Death Discovery

Fig. 3: CUL4B degrades the FBP1 protein in the nucleus.

From: MLN4924 suppresses tumor metabolism and growth of clear cell renal cell carcinoma by stabilizing nuclear FBP1

Fig. 3

A Screening the Cullin-RING E3 ubiquitin ligase of FBP1 by transfecting Flag-FBP1 and Myc-Cullins as indicated. Immunoprecipitation and Western blot analysis confirmed the interaction between FBP1 and CUL4B. B Co-localization of FBP1 and CUL4B in 786-O cells was detected by immunofluorescence. C Western blot was performed to assess the cytoplastic and nuclear expression of FBP1 after transfection with CUL4B plasmids. D Expression of CUL4B was identified in wild-type (WT) 786-O cells and two distinct CUL4B knockout 786-O cell lines by Western blot. E Western blot was performed to assess the cytoplasmic and nuclear expression of FBP1 after CUL4B knockout. F Immunoprecipitation and Western blot with the indicated antibodies were performed to determine the level of FBP1 ubiquitylation in WT 786-O cells and two distinct CUL4B knockout cell lines. G sgControl and sgCUL4B 786-O cells were incubated with 100 μg/mL CHX at the indicated time points for Western blot analysis.

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