Fig. 4: MLN4924 inhibits downstream effectors of HIF and correlates with tumor metabolism. | Cell Death Discovery

Fig. 4: MLN4924 inhibits downstream effectors of HIF and correlates with tumor metabolism.

From: MLN4924 suppresses tumor metabolism and growth of clear cell renal cell carcinoma by stabilizing nuclear FBP1

Fig. 4

A Western blot was performed to assess the protein expression levels of HIF-2α after treated with MLN4924 (1 μM). B Western blot was performed to assess the protein expression levels of HIF-2α after transfection with CUL4B plasmids. C GLUT1, LDHA, PDK1 and VEGF mRNA levels were quantified by qPCR after overexpressed of CUL4B in 786-O cells. D WT and CUL4B knockout 786-O cells were used to measure GLUT1, LDHA, PDK1 and VEGF mRNA levels. E GLUT1, LDHA, PDK1 and VEGF mRNA levels were quantified by qPCR with or without MLN4924 treatment in 786-O cells. F Lactate, glucose uptake and NADPH levels were measured after overexpressed of CUL4B in 786-O cells. G WT and CUL4B knockout 786-O cells were used to measure lactate, glucose uptake and NADPH levels. H Lactate, glucose uptake and NADPH levels were measured with or without MLN4924 treatment in 786-O cells. I Lactate, glucose uptake and NADPH levels were measured with or without HIF-2α inhibitor treatment in 786-O cells. HIFi, HIF-2α inhibitor. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001.

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