Fig. 5: Sotorasib promotes the accumulation of NEU1 by blocking its ubiquitination degradation. | Cell Death Discovery

Fig. 5: Sotorasib promotes the accumulation of NEU1 by blocking its ubiquitination degradation.

From: Sotorasib-impaired degradation of NEU1 contributes to cardiac injury by inhibiting AKT signaling

Fig. 5

A CCC-HEH-2 were treated with 20 μM sotorasib for 0, 6, 9, 12, or 24 h and exposed to different concentrations of 0, 10, 15, or 20 μM sotorasib for 24 h. CCC-HEH-2 NEU1mRNA level. (n = 3). B, C CHX (10 μg/mL), MG132 (10 μM), and /or sotorasib (20 μM) treated 0, 1, 2, 4, 6, 8 h. NEU1 protein expression level. D 293T cells were transfected with Ub-HA and/or NEU1-Flag and sotorasib was treated for 48 h. HA, Flag expression levels in 293T cells (n = 3). E CETSA, NEU1 expression level was verified in 293T cells. Data expressed as mean ± SD, ns, no significance.

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