Fig. 2: Tumor-promoting capability of CAFs demonstrates variability. | Cell Death Discovery

Fig. 2: Tumor-promoting capability of CAFs demonstrates variability.

From: Exosomes derived from ccRCC cells confers fibroblasts activation to foster tumor progression through Warburg effect by downregulating PANK3

Fig. 2

A Electron microscopy and Nanosight particle tracking analysis of exosomes derived from CAFs. Scale bar, 100 nm. B Western blotting assay of indicated proteins in exosomes from CAFs. C Migration assays of SN12 treated with equal quantities of exosomes derived from CAFs. Scale bar, 100 μm. D Immunoblotting assays of EMT markers in SN12 treated with equal quantities of exosomes derived from CAFs. E Xenograft assays of SN12 with indicated treatments were performed on nude mice. Representative tumors (left) and tumor volumes (right) were shown. F Representative images and quantitative analysis of lung metastasis of indicated mice treated with exosomes derived from CAFs or blank control were determined by luciferase-based bioluminescence imaging. G HE and IHC staining of FAP on lung metastatic tissue samples of indicated nude mice. Scale bar, 50 μm. Each experiment was performed three times independently and results are presented as mean ± s.d. Student’s t-test was used to analyze the data (*p < 0.05; **p < 0.01; ***p < 0.001).

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