Fig. 3: CAFs undergo the Warburg effect in response to activation. | Cell Death Discovery

Fig. 3: CAFs undergo the Warburg effect in response to activation.

From: Exosomes derived from ccRCC cells confers fibroblasts activation to foster tumor progression through Warburg effect by downregulating PANK3

Fig. 3

A Transcriptome sequencing of differentially expressed genes between CAFs and NFs. B GO pathway enrichment analysis of differentially expressed genes. C KEGG pathway enrichment analysis of differentially expressed genes. D Transmission electron microscopy images of NFs and CAFs. Scale bar, 50 μm. E Analysis of ECAR and OCR between CAF3 and NF. F Comparison of glucose uptake, GLUT1 expression, mitochondrial ROS level, and lactate production between NF and CAF3. G Detection of lactic acid in indicated conditioned medium. H Immunoblotting assays of MCTs in NFs and SN12 with indicated treatments. I Immunoblotting assays of MCT1 expression in NFs transfected with siRNAs targeting MCT1 or control. J Xenograft assays of SN12 with indicated treatments were performed on nude mice. Representative tumors (left) and tumor volumes (right) were shown. Each experiment was performed three times independently and results are presented as mean ± s.d. Student’s t-test was used to analyze the data (*p < 0.05; **p < 0.01; ***p < 0.001).

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