Fig. 2: Reduced growth and cell death caused by extracellular ammonia is not coupled to ATR/ATM checkpoint signalling.
From: Low extracellular pH protects cancer cells from ammonia toxicity
A A549 cells were cultured for three days in the presence of indicated concentrations of NH4Cl. Cells were then analysed by flow cytometry. SSC; side scatter, n = 20.000). B A549 cells were cultured in media with the indicated concentrations of NH4Cl and then imaged in vivo by phase-contrast microscopy. C Cell death assay. After indicated cell lines had been cultured in media with indicated concentrations of NH4Cl for three days, cells were collected by trypsination, stained with propidium iodide, and analysed by flow cytometry (n = 20000). D A549 cells were cultured for three days in media with indicated concentrations of NH4Cl. Whole-cell lysates were prepared, and Western blot analysis was performed. Ponceau S was used as a loading and blotting control. C1 and C2 represent control lysates. C1; A549 cells cultured overnight in the presence of 50 μM Etoposide. C2; Jurkat cells subjected to heat shock (10 minutes at 44 °C followed by 6 hours at 37 °C). Molecular markers and densitometry analysis are shown in the Source Data file.
