Fig. 3: Mitochondrial Morphology and Function analysis of OPA1 Mutations.
From: Contrasting pathophysiological mechanisms of OPA1 mutations in autosomal dominant optic atrophy
A, B Mitochondrial morphology in HeLa and RGC5 cells overexpressing OPA1 mutants. Mitochondria were marked by red fluorescence. The cell nucleus was stained by DAPI. C–F Quantitative analysis of mitochondrial morphology classified as filamentous (white), intermediate (gray), and fragmented (black). The HeLa and RGC5 cells transfected with the c.1034 G > A mutant displayed a fragmented mitochondrial network. G, H Colocalization between cytochrome (cyt) c (green fluorescence) and mitochondrial in HeLa and RGC5 cells overexpressing OPA1 mutants. The transfected cells have been indicated with stars. I–L Quantitative analysis of colocalization coefficients of cyt c and mitochondria. The cells transfected with the c.1034 G > A mutant displayed a decreased coefficient. Asterisks indicate statistical significance (*adjusted p < 0.05, **adjusted p < 0.01, ***adjusted p < 0.001).
