Fig. 5: OPA1 Expression Level related analysis.
From: Contrasting pathophysiological mechanisms of OPA1 mutations in autosomal dominant optic atrophy
A Western blot of the OPA1 isoforms and expression level in HeLa and RGC5 cells overexpressing different OPA1 mutants. The two OPA1 isoforms were tagged by flag. B, C Quantitative analysis of the expression level of OPA1 mutant proteins. The expression level of OPA1 mutant proteins decreased significantly. D–G OPA1 mRNA expression levels in blood samples from ADOA family members. Unaffected family members are show in white, mildly affected family members in light purple, and the proband in dark blue. H Mitochondrial morphology in HeLa cells transfected with different concentrations of OPA1 siRNA. I OPA1 mRNA expression level detection by qPCR. J, K Quantification of OPA1 with Western blot. L, M Quantitative analysis of mitochondrial morphology. Loss of OPA1 induced fragmentation of the mitochondrial network. N, O Western blot of the c-cas3 expression level in HeLa cells transfected with different concentrations of OPA1 siRNA. Decreased OPA1 led to mitochondrial fragmentation with a significant increase in apoptosis.
