Fig. 3: TRPV1 expression increases in the testis on day 9 after cryptorchidism.

A, B TRPV1 mRNA and protein expression in the testis on day 9 after cryptorchidism (n = 3). A mRNA expression was assessed using RT-qPCR. Amplification of β-actin mRNA was used as an internal control. B Protein expression was determined using Western blot. Protein expression levels were normalized to β-actin and densitometric analyses were performed with ImageJ software. C IF staining was applied to detect TRPV1 (red) in the 9-day cryptorchid testis. Dashed boxes showed localization of the enlarged images. White arrows indicated cells with strong positive signals. Data were represented as mean ± SD from three independent experiments. **p < 0.01 by Student’s t test.