Fig. 2: Knockdown of CXCR3 reduces tumor cell proliferation.
A MTT assays of U87 and U251 cells with CXCR3 knockdown (shCXCR3) from day 1 to day 4 compared to control (shCtrl). B EdU incorporation assay showed decreased U87 EdU+ proliferating cells following CXCR3 knockdown measured by flow cytometry (left). Quantification of signal intensity (right). C. Representative fluorescence images of EdU stained U87 and U251 cells. EdU (red); DAPI (blue). Original magnification ×40 D. Representative images (top) and quantification of colonies (bottom) formed after 14 days of incubation. E Representative images of wound simulation assay after 12 hours of incubation. Original magnification: ×4; Scale bar: 100 mm. F, G Representative images and tumor weight of U87 subcutaneous xenografts from nude mice at day 28 after tumor injection (n = 6). H, I Representative image and quantification of bioluminescence intensity in U87 orthotopic xenograft expressing shCXCR3 (n = 5) compared to shCtrl control (n = 4). The bioluminescence signal was measured twice per week at indicated intervals. Photon flux (p/s/cm2/sr) was normalized at day 1 and presented as fold change at day 14, 17, 21, 24, and 28. J. Representative H&E staining of orthotopic implanted U87 cells after mice sacrifice at day 28 at experimental endpoint, Scale bar 800 μm. Error bars indicate mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns (no statistical significance). G, I Error bars indicate mean ± SEM.
