Fig. 2: The LTB4-BLT1 axis attenuates IL-1β production in macrophages following influenza infection.
From: The LTB4-BLT1 axis attenuates influenza-induced lung inflammation by suppressing NLRP3 activation
A Expression levels of BLT1 and BLT2 were determined in BMDMs with or without IAV infection by reverse transcription qPCR at 16 h post-infection (n = 6 per group). (representative of three assays). B Expression levels of BLT1 and BLT2 were determined in MLE-12 with or without IAV infection by reverse transcription qPCR at 24 h post infection (n = 6 per group). (representative of three assays). C The LTB4 level in the BMDMs and MLE-12 cell culture supernatants was assessed by ELISA at 16 h (BMDMs) and 24 h (MLE-12) post infection (n = 6 per group). (representative of three assays). D–F, BMDMs isolated from WT and BLT1−/− mice were primed with or without 100 nM LTB4 0.5 h, followed by infection with IAV (MOI = 20) for 16 h. D Cell supernatants and whole cell lysis were collected for immunoblot analysis. IL-1β (E) and IL-18 (F) release in the supernatants was determined by ELSIA, respectively. (representative of three assays). Statistics: mean ± SD; unpaired, two-tailed Student’s t test (A–C); two-way ANOVA (E, F); *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.
