Fig. 2: APOE4 impairs the phagocytic ability in RCMs and BMDMs. | Cell Death Discovery

Fig. 2: APOE4 impairs the phagocytic ability in RCMs and BMDMs.

From: APOE4 impairs macrophage lipophagy and promotes demyelination of spiral ganglion neurons in mouse cochleae

Fig. 2

A Immunofluorescent assay of RCMs labelled with MBP (green) and F4/80 (red) in the cochleae of 10-month-old APOE4 and APOE3 mice. Scale bar: 100 μm. Dashed frames denote the enlarged region showing the colocalization of MBP and F4/80. Scale bar: 50 μm. B Quantification of MBP fluorescence intensity per cell (20 RCMs in every view, each dot represents all RCMs in a microscope field, n = 6; **P < 0.01, ***P < 0.001 by two-way ANOVA). C The proportion of MBP+ RCMs (20 RCMs in every view, each dot represents all RCMs in a microscope field, n = 6; **P < 0.01, ****P < 0.0001 by two-way ANOVA). D Immunofluorescent assay of APOE3 and APOE4 BMDMs labelled with MBP (green) and F4/80 (red). Scale bar: 100 μm. Dashed frames denote the enlarged region showing the colocalization of MBP and F4/80. Scale bar: 50 μm. E Quantification of MBP fluorescence intensity per cell (50 BMDMs in every view, each dot represents all BMDMs in a microscope field, n = 6; *P < 0.05, ****P < 0.0001 by two-way ANOVA). F The proportion of MBP-positive (MBP+) BMDMs (50 BMDMs in every view, each dot represents all BMDMs in a microscope field, n = 6; ***P < 0.001, ****P < 0.0001 by two-way ANOVA).

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