Fig. 4: APOE4 disrupted cholesterol homoeostasis in RCMs and BMDMs. | Cell Death Discovery

Fig. 4: APOE4 disrupted cholesterol homoeostasis in RCMs and BMDMs.

From: APOE4 impairs macrophage lipophagy and promotes demyelination of spiral ganglion neurons in mouse cochleae

Fig. 4

A Flow cytometry assay of cholesterol in APOE3/APOE4 RCMs and BMDMs. BMDMs and RCMs were stained using CD45high, CD11bhigh and F4/80+ as the macrophage markers. B Percentage of RCMs and BMDMs with high cholesterol content (n = 3, *P < 0.05 by one-way ANOVA, BMDMs: n = 3, **P < 0.01 by one-way ANOVA). C Number of LDs per cell in APOE3 and APOE4 BMDMs untreated (Ctrl) or treated with cholesterol (+Cholesterol) and Myelin debris (+Mye) (n = 6). D Immunofluorescent assay of LDs (BODIPY in green) in APOE3 and APOE4 BMDMs (F4/80 in red) untreated (Ctrl) or treated with cholesterol (+Cholesterol) and Myelin debris (+Mye). Scale bar: 100 μm.

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