Fig. 5: Impaired autophagy in APOE4 BMDMs was partially rescued by trehalose treatment. | Cell Death Discovery

Fig. 5: Impaired autophagy in APOE4 BMDMs was partially rescued by trehalose treatment.

From: APOE4 impairs macrophage lipophagy and promotes demyelination of spiral ganglion neurons in mouse cochleae

Fig. 5

A Expression of GLUT8 in BMDMs and cochleae of 10-month-old APOE4 and APOE3 mice. The expression of GLUT8 was significantly decreased in APOE4 RCMs and BMDMs compared with APOE3 controls. B Expression of LC3 and SQSTM1/P62 in APOE3 and APOE4 BMDMs. Cells were treated with myelin debris (+Mye) or were co-treated with both myelin debris and trehalose (+Mye/Tre). The ratio of LC3II/LC3I was significantly decreased and SQSTM1/P62 was upregulated in trahalose-treated APOE4 and APOE3 BMDMs (+Mye), which could be reversed by the co-treatment of trehalose (+Mye/Tre). C The ratio of LC3II/LC3I in APOE3 and APOE4 BMDMs (n = 6, ns not significant, ***P < 0.001, ****P < 0.0001 by two-way ANOVA). D Immunofluorescence staining of LDs (BODIPY in green) in APOE3 and APOE4 BMDMs (LC3 in red). Scale bar: 50 μm. Dashed frames denote the enlarged region in the panel below. Scale bar: 25 μm. Cells were treated with myelin debris (+Mye) or were co-treated with both myelin debris and trehalose (+Mye/Tre). E Average number of LDs per BMDM (10 BMDMs in every view, each dot represents all BMDMs in a microscope field, n = 6; ns not significant, **P < 0.01, ***P < 0.001 by two-way ANOVA). F LC3-positive (LC3+) puncta per BMDM (10 BMDMs in every view, each dot represents all BMDMs in a microscope field, n = 6; ns not significant, *P < 0.05; **P < 0.01 by two-way ANOVA). G Portion of LC3-positive (LC3+) LDs in APOE4 and APOE3 BMDMs (100 LDs in every view, each dot represents all LDs in a microscope field, n = 6, ns not significant, *P < 0.05; ***P < 0.001 by two-way ANOVA).

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