Fig. 7: RNF144A-VRK2-G3BP1 axis sensitizes osteosarcoma cell to stress.
From: RNF144A-VRK2-G3BP1 axis regulates stress granule assembly
A, B The cell viability of pNTAP-VRK2 stably expressing U2OS cells was measured through CCK-8 assay. The stress was induced with 50 μM of SA (A) or 50 μM of cisplatin (B) for 24 h or 48 h (n = 6, n = 8, respectively). Representative image (C) and the quantification (D) of colony formation assay performed with pNTAP-VRK2 stably expressing U2OS cells. pNTAP-VRK2 stably expressing U2OS cells were treated with SA (50 μM) from 4th day to 7th day (n = 3). Representative image (E) and the quantification (F) of colony formation assay performed with pNTAP-VRK2 stable U2OS cells. pNTAP-VRK2 stably expressing U2OS cells were treated with cisplatin (25 μM) from 4th day to 7th day (n = 3). n.s., not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; two-way ANOVA with Tukey’s multiple comparison test was performed for (A, B, D, F). The “n” represents the number of independent experiments. Error bars indicate SDs.
