Fig. 2: Skp2 knockdown enhances IR-induced OSCC-intrinsic apoptosis.
From: E3 ligase Skp2-mediated stabilization of survivin contributes to radioresistance
A, B Skp2 knockdown CAL27 cells (A) and SCC25 cells (B) were assayed for cell viability by MTS after IR (2 Gy) treatment. ***p < 0.001. C, D Skp2 knockdown CAL27 cells (C) and SCC25 cells (D) were assayed for colony-forming ability by soft agar colony formation assay after IR (2 Gy) treated/untreated. ***p < 0.001. Scale bar, 200 μm. E, F Cell viability of shSkp2 CAL27 cells (E) /shSkp2 SCC25 cells (F) was assayed by MTS using apoptosis inhibitor (z-VAD-fmk) treatment. ***p < 0.001. G, H Skp2-knockdown CAL27 cells treated with/without IR (2 Gy) after 72 h, subcellular fractions (G) and whole cell extracts (H) were isolated for IB analysis. I, J Caspase 3 activity was examined by caspase 3 assay kit in CAL27 cells (I) and SCC25 cells (J). ***p < 0.001.
