Fig. 3: Skp2 deletion and IR treatment reduce Survivin protein levels.
From: E3 ligase Skp2-mediated stabilization of survivin contributes to radioresistance
A Skp2 deficient OSCC cells were treated with/without IR and cultured for 24 h followed by IB assay. B–D CAL27 and SCC25 cells were transiently transfected with siRNA targeting Survivin for 24 h after transfection, followed by IR treatment and cultured for 24 h. B IB assay to detect the protein expression of Survivin. C MTS assay to detect cell viability. ***p < 0.001. D Caspase 3 activity was detected in CAL27 (left) and SCC25 (right) cells using caspase 3 assay kit. ***p < 0.001. E–G Flag-Skp2 was transfected in Skp2 knockdown CAL27 cells for 48 h, then treated with IR and cultured for 24 h. Protein expression levels of Skp2 and Survivin were detected by IB (E), cell viability was detected by MTS assay (F) and colony formation ability of OSCC cells was detected by colony formation assay (G). ***p < 0.001.
