Fig. 9: Skp2 inhibitor use partially overcomes radiotherapy resistance in OSCC cells.
From: E3 ligase Skp2-mediated stabilization of survivin contributes to radioresistance
A–D CAL27-IR and SCC25-IR cells were subjected to IR, SZL PI-41, or co-treatment and were assayed for cell viability using MTS (A), colony formation using soft agar colony formation assay (B), Scale bar, 200 μm), caspase 3 activity using caspase 3 assay kit (C), and IB for cleaved-caspase 3 protein expression level (D). ***p < 0.001. E–G CAL27-IR cell-derived tumors were treated with IR, SZL PI-41 or combination therapy. Tumor volume (E) and mouse survival time (G) were recorded. Tumor tissues were collected and stained with IHC (F, Scale bar, 20 μm). **P < 0.01, ***P < 0.001.
