Fig. 3: MYC exerts G0/G1 phase arrest via the transcriptional regulation of P21 and P27. | Cell Death Discovery

Fig. 3: MYC exerts G0/G1 phase arrest via the transcriptional regulation of P21 and P27.

From: The dual HDAC/PI3K inhibitor CUDC-907 inhibits the growth and proliferation of MYC-driven Group 3 medulloblastoma

Fig. 3

A Data mining according to R2 Genomics Analysis and Visualization Platform revealed that MYC is closely related to the cell cycle. B Flow cytometry analysis revealed that the proportion of G0/G1 phase among MB cells treated with CUDC-907 increased with increasing drug concentration. C Western blot was used to detect the expression levels of G0/G1 related cyclin-dependent kinases (CDKs) and cyclins. D The expression of upstream CDK inhibitors (CKIs). E CUDC-907 enhanced the fluorescence activity of plasmids carrying the P21 and P27 promoters. F MYC was overexpressed in MB cells. G Overexpression of MYC weakened the G0/G1 phase blockade induced by CUDC-907. H Western blot detection of G0/G1 phase protein level. I Dual-luciferase assays revealed a relative decrease in the fluorescence activity of P21 and P27 after the overexpression of MYC. NS p > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001.

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