Fig. 3: UVB-induced cell death is promoted by RIPK3. | Cell Death Discovery

Fig. 3: UVB-induced cell death is promoted by RIPK3.

From: UVB-Induced necroptosis of the skin cells via RIPK3-MLKL activation independent of RIPK1 kinase activity

Fig. 3

Western blotting of phosphorylated (p)-RIPK3, total RIPK3 (A), phosphorylated (p)-MLKL and total MLKL (B) in HaCaT cells at 3 h, 6 h, 12 h and 24 h following TSZ treatment or 60 mJ/cm2 UVB irradiation. C Cell viability of HaCaT and 10 µM GSK’872-pretreated HaCaT cells 24 h after 60 mJ/cm2 UVB irradiation. D Cell viability of NC HaCaT, RIPK3-KD HaCaT and RIPK3-OE cells 24 h after 60 mJ/cm2 UVB irradiation. Viability in both (C) and (D) determined based on ATP levels. E PI fluorescent staining of HaCaT, GSK’872 pretreated HaCaT, RIPK3-KD HaCaT and RIPK3-OE HaCaT cells at 24 h after 60 mJ/cm2 UVB irradiation; red staining indicating dead cells only (scale bar: 100 μm). F Apoptosis rates of HaCaT and GSK’872-pretreated HaCaT cells at 24 h after 60 mJ/cm2 UVB irradiation. G Apoptosis rates of NC HaCaT, RIPK3-KD HaCaT and RIPK3-OE HaCaT cells 24 h after 60 mJ/cm2 UVB irradiation. All TSZ treatment serving as positive control. RIPK3-KD: RIPK3 knockdown; RIPK3-OE: RIPK3 overexpression. Data are presented as mean ± SD, n = 3. P values determined by one-way analysis of variance (ANOVA), *P < 0.05, **P < 0.01.

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