Fig. 4: UVB-induced inflammation is mediated by RIPK3 through necroptosis, independent of RIPK1 kinase activity. | Cell Death Discovery

Fig. 4: UVB-induced inflammation is mediated by RIPK3 through necroptosis, independent of RIPK1 kinase activity.

From: UVB-Induced necroptosis of the skin cells via RIPK3-MLKL activation independent of RIPK1 kinase activity

Fig. 4

A Western blot analysis of p-RIPK3, RIPK3, p-MLKL and MLKL in HaCaT cells pretreated with varying concentrations of Nec-1, assessed 12 h post-UVB irradiation. B Western blot analysis of p-MLKL and MLKL in HaCaT cells pretreated with 10 or 50 μM GSK’872, assessed 12 h post-UVB irradiation. C Levels of IL-1β, TNF-α and IL-6 in the medium detected by ELISA at 12 h after irradiation of HaCaT and GSK’872 pretreated HaCaT cells with 60 mJ/cm2 UVB. D Levels of IL-1β, TNF-α and IL-6 in the medium detected by ELISA at 12 h after irradiation of NC HaCaT, RIPK3-KD HaCaT and RIPK3-OE HaCaT cells with 60 mJ/cm2 UVB. E Western blot analysis of p-p65 and total p65 in NC HaCaT and RIPK3-KD HaCaT cells 12 h after 60 mJ/cm2 UVB irradiation. TSZ and LPS treatments serving as positive controls. Data are presented as mean ± SD, n = 3. P values determined by one-way analysis of variance (ANOVA), *P < 0.05, **P < 0.01.

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