Fig. 3: FABP3 is a key downstream target of MGP-mediated adipogenic differentiation of MSCs.

A Cluster heatmap, B Volcano map and C Venn diagram for MSCs infected with sh-NC or sh-MGP lentivirus on the 3rd day of adipogenic differentiation are shown. D After MSCs were infected with sh-NC, shMGP, OE-NC, or OE-MGP lentiviruses, the relative RNA expression of FABP3 was determined via qRT–PCR. E ORO staining and quantification of MSCs infected with sh-NC, sh-FABP3, OE-NC or OE-FABP3 lentivirus on the 10th day to assess adipogenic differentiation. F Protein levels of FABP3, MGP and the adipogenic markers C/EBP-α, LPL and Perilipin 1 in MSCs infected with sh-NC, sh-FABP3, OE-NC or OE-FABP3 lentiviruses on the 5th day were detected. G, H The protein and relative RNA expression levels of MGP, FABP3, and the adipogenic markers C/EBP-α, LPL and Perilipin 1 after FABP3 knockdown or/and MGP overexpression using lentiviruses were determined via Western blotting analysis and qRT–PCR. I ORO staining and quantification were performed after 10 days to assess adipogenic differentiation in cells with FABP3 knockdown and/or MGP overexpression. Scale bar = 50 μm. All the data are presented as the means ± SDs; n = 6 per group. Student’s t test or ANOVA was used to determine significant differences. ns not statistically significant; *P < 0.05; **P < 0.01; and ***P < 0.001.